Research Paper PRODUCTION AND PURIFICATION AND CHARACTERIZATION OF STREPTOKINASE USING Bacillus licheniformis UNDER SOLID STATE FERMENTATION

Streptokinase is a novel fibrionotic protein produced by several bacterial species. As a therapeutic, streptokinase can be used as thromboembolic disorders where it dissolves a blood clot by the activation of plasminogen to plasmin. In the present study, Bacillus licheniformis used for the production of streptokinase under soya bean as carbon source. The optimum day, pH and temperature were also optimized and were found to be 48hours, pH of 7 and temperature at 400C respectively. The enzyme was partially purified using ammonium sulphate followed by dialysis membrane and sephadexG-100 column chromatography. The molecular weight of the streptokinase was determined using SDS-PAGE and was found to be 63 kDa. The enzyme was further confirmed its Proteolytic activity and thrombolytic activity.